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c3ar  (Hycult Biotech)
94
Hycult Biotech c3ar
Reactive astrocytes, tau and Aβ pathology in Tg Casp3 mice. A Stereological cell count of GFAP + cells in the hippocampus (scale: 50 µm) of Tg Sham and Tg Casp3 male mice ( n = 4 mice/group; Mann–Whitney test: * p = 0.029). Nuclei are counterstained with DAPI. B IL-18R and GFAP immunostaining (scale: 15 µm), and plot showing % of IL-18R + /GFAP + cells (Tg Sham: n = 6, Tg Casp3: n = 4 mice; Unpaired t-test: **** p < 0.0001). C p-NFκB and S100β immunostaining (scale: 15 µm) and plot showing % of p-NFκB + /S100β + cells in hippocampus (Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t-test: **** p < 0.0001). D Complement C3 and GFAP immunostaining (scale: 10 µm), and plot showing C3 levels ( n = 5 mice/group; Unpaired t -test: ** p = 0.001). E Complement <t>C3aR</t> and DAPI (scale: 20 µm) or Iba1 immunostaining (scale: 20 µm, inset 10 µm), and plots showing C3aR levels on neurons ( n = 4 mice/group; Unpaired t -test: *** p = 0.0007) and microglia (Unpaired t -test: *** p = 0.0003). F Representative western blots and plot showing levels of p-GSK3β/GSK3β (as % of Tg Sham). β-Tubulin was used as loading control ( n = 6 mice/group; Unpaired t -test: * p = 0.048). G AT8 immunostaining in WT, Tg Sham and Tg Casp3 hippocampi and plot showing AT8 + area on total hippocampal area (scale bar: 250 µm; n = 5 mice/group. One-Way ANOVA: F 2,12 = 8.817; p = 0.004. WT Sham vs Tg Casp3: * p = 0.011, Tg Sham vs Tg Casp3: ** p = 0.007, with Tukey’s). The insets and bottom plot show AT8-reactive cells and AT8 levels (scale: 20 µm; n = 5 mice/group. One-Way ANOVA: F 2,12 = 19.27; p = 0.0002. WT vs Tg Casp3: *** p = 0.0003, Tg Sham vs Tg Casp3: *** p = 0.0007, with Tukey’s). H MAP2 immunostaining and plot showing MAP2 levels (counterstained with NeuroTrace; scale: 25 µm; Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t -test: * p = 0.017). I 6E10 immunostaining and plot of intracellular Aβ levels (Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t -test: ** p = 0.002). Nuclei are counterstained with DAPI (scale: 50 µm). J) Immunostaining for Aβ plaques and Iba1 (scale: 100 µm, inset 20 µm), and plot reporting hippocampal Aβ plaque load ( n = 7 mice/group; Mann–Whitney test: * p = 0.021). K DA and 5-HT loss due to midbrain lesion triggers microglia-astrocyte-neuron crosstalk fostering neuroinflammation and, thus, exacerbating AD pathology in the hippocampus of Tg Casp3 mice. [Figure created using BioRender.com]
C3ar, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+c3ar/pmc12516900-103-19-21?v=Hycult+Biotech
Average 94 stars, based on 1 article reviews
c3ar - by Bioz Stars, 2026-08
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c3ar1 rat anti mouse c3ar ab hycult biotech  (Hycult Biotech)
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Reactive astrocytes, tau and Aβ pathology in Tg Casp3 mice. A Stereological cell count of GFAP + cells in the hippocampus (scale: 50 µm) of Tg Sham and Tg Casp3 male mice ( n = 4 mice/group; Mann–Whitney test: * p = 0.029). Nuclei are counterstained with DAPI. B IL-18R and GFAP immunostaining (scale: 15 µm), and plot showing % of IL-18R + /GFAP + cells (Tg Sham: n = 6, Tg Casp3: n = 4 mice; Unpaired t-test: **** p < 0.0001). C p-NFκB and S100β immunostaining (scale: 15 µm) and plot showing % of p-NFκB + /S100β + cells in hippocampus (Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t-test: **** p < 0.0001). D Complement C3 and GFAP immunostaining (scale: 10 µm), and plot showing C3 levels ( n = 5 mice/group; Unpaired t -test: ** p = 0.001). E Complement <t>C3aR</t> and DAPI (scale: 20 µm) or Iba1 immunostaining (scale: 20 µm, inset 10 µm), and plots showing C3aR levels on neurons ( n = 4 mice/group; Unpaired t -test: *** p = 0.0007) and microglia (Unpaired t -test: *** p = 0.0003). F Representative western blots and plot showing levels of p-GSK3β/GSK3β (as % of Tg Sham). β-Tubulin was used as loading control ( n = 6 mice/group; Unpaired t -test: * p = 0.048). G AT8 immunostaining in WT, Tg Sham and Tg Casp3 hippocampi and plot showing AT8 + area on total hippocampal area (scale bar: 250 µm; n = 5 mice/group. One-Way ANOVA: F 2,12 = 8.817; p = 0.004. WT Sham vs Tg Casp3: * p = 0.011, Tg Sham vs Tg Casp3: ** p = 0.007, with Tukey’s). The insets and bottom plot show AT8-reactive cells and AT8 levels (scale: 20 µm; n = 5 mice/group. One-Way ANOVA: F 2,12 = 19.27; p = 0.0002. WT vs Tg Casp3: *** p = 0.0003, Tg Sham vs Tg Casp3: *** p = 0.0007, with Tukey’s). H MAP2 immunostaining and plot showing MAP2 levels (counterstained with NeuroTrace; scale: 25 µm; Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t -test: * p = 0.017). I 6E10 immunostaining and plot of intracellular Aβ levels (Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t -test: ** p = 0.002). Nuclei are counterstained with DAPI (scale: 50 µm). J) Immunostaining for Aβ plaques and Iba1 (scale: 100 µm, inset 20 µm), and plot reporting hippocampal Aβ plaque load ( n = 7 mice/group; Mann–Whitney test: * p = 0.021). K DA and 5-HT loss due to midbrain lesion triggers microglia-astrocyte-neuron crosstalk fostering neuroinflammation and, thus, exacerbating AD pathology in the hippocampus of Tg Casp3 mice. [Figure created using BioRender.com]
C3ar1 Rat Anti Mouse C3ar Ab Hycult Biotech, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+c3ar/pm37861348-77-16-21?v=Hycult+Biotech
Average 93 stars, based on 1 article reviews
c3ar1 rat anti mouse c3ar ab hycult biotech - by Bioz Stars, 2026-08
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rat anti mouse c3ar  (Hycult Biotech)
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Hycult Biotech rat anti mouse c3ar
Reactive astrocytes, tau and Aβ pathology in Tg Casp3 mice. A Stereological cell count of GFAP + cells in the hippocampus (scale: 50 µm) of Tg Sham and Tg Casp3 male mice ( n = 4 mice/group; Mann–Whitney test: * p = 0.029). Nuclei are counterstained with DAPI. B IL-18R and GFAP immunostaining (scale: 15 µm), and plot showing % of IL-18R + /GFAP + cells (Tg Sham: n = 6, Tg Casp3: n = 4 mice; Unpaired t-test: **** p < 0.0001). C p-NFκB and S100β immunostaining (scale: 15 µm) and plot showing % of p-NFκB + /S100β + cells in hippocampus (Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t-test: **** p < 0.0001). D Complement C3 and GFAP immunostaining (scale: 10 µm), and plot showing C3 levels ( n = 5 mice/group; Unpaired t -test: ** p = 0.001). E Complement <t>C3aR</t> and DAPI (scale: 20 µm) or Iba1 immunostaining (scale: 20 µm, inset 10 µm), and plots showing C3aR levels on neurons ( n = 4 mice/group; Unpaired t -test: *** p = 0.0007) and microglia (Unpaired t -test: *** p = 0.0003). F Representative western blots and plot showing levels of p-GSK3β/GSK3β (as % of Tg Sham). β-Tubulin was used as loading control ( n = 6 mice/group; Unpaired t -test: * p = 0.048). G AT8 immunostaining in WT, Tg Sham and Tg Casp3 hippocampi and plot showing AT8 + area on total hippocampal area (scale bar: 250 µm; n = 5 mice/group. One-Way ANOVA: F 2,12 = 8.817; p = 0.004. WT Sham vs Tg Casp3: * p = 0.011, Tg Sham vs Tg Casp3: ** p = 0.007, with Tukey’s). The insets and bottom plot show AT8-reactive cells and AT8 levels (scale: 20 µm; n = 5 mice/group. One-Way ANOVA: F 2,12 = 19.27; p = 0.0002. WT vs Tg Casp3: *** p = 0.0003, Tg Sham vs Tg Casp3: *** p = 0.0007, with Tukey’s). H MAP2 immunostaining and plot showing MAP2 levels (counterstained with NeuroTrace; scale: 25 µm; Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t -test: * p = 0.017). I 6E10 immunostaining and plot of intracellular Aβ levels (Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t -test: ** p = 0.002). Nuclei are counterstained with DAPI (scale: 50 µm). J) Immunostaining for Aβ plaques and Iba1 (scale: 100 µm, inset 20 µm), and plot reporting hippocampal Aβ plaque load ( n = 7 mice/group; Mann–Whitney test: * p = 0.021). K DA and 5-HT loss due to midbrain lesion triggers microglia-astrocyte-neuron crosstalk fostering neuroinflammation and, thus, exacerbating AD pathology in the hippocampus of Tg Casp3 mice. [Figure created using BioRender.com]
Rat Anti Mouse C3ar, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+c3ar/pm37443787-79-40-46?v=Hycult+Biotech
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rat anti mouse c3ar - by Bioz Stars, 2026-08
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rat anti c3ar  (Hycult Biotech)
94
Hycult Biotech rat anti c3ar
Reactive astrocytes, tau and Aβ pathology in Tg Casp3 mice. A Stereological cell count of GFAP + cells in the hippocampus (scale: 50 µm) of Tg Sham and Tg Casp3 male mice ( n = 4 mice/group; Mann–Whitney test: * p = 0.029). Nuclei are counterstained with DAPI. B IL-18R and GFAP immunostaining (scale: 15 µm), and plot showing % of IL-18R + /GFAP + cells (Tg Sham: n = 6, Tg Casp3: n = 4 mice; Unpaired t-test: **** p < 0.0001). C p-NFκB and S100β immunostaining (scale: 15 µm) and plot showing % of p-NFκB + /S100β + cells in hippocampus (Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t-test: **** p < 0.0001). D Complement C3 and GFAP immunostaining (scale: 10 µm), and plot showing C3 levels ( n = 5 mice/group; Unpaired t -test: ** p = 0.001). E Complement <t>C3aR</t> and DAPI (scale: 20 µm) or Iba1 immunostaining (scale: 20 µm, inset 10 µm), and plots showing C3aR levels on neurons ( n = 4 mice/group; Unpaired t -test: *** p = 0.0007) and microglia (Unpaired t -test: *** p = 0.0003). F Representative western blots and plot showing levels of p-GSK3β/GSK3β (as % of Tg Sham). β-Tubulin was used as loading control ( n = 6 mice/group; Unpaired t -test: * p = 0.048). G AT8 immunostaining in WT, Tg Sham and Tg Casp3 hippocampi and plot showing AT8 + area on total hippocampal area (scale bar: 250 µm; n = 5 mice/group. One-Way ANOVA: F 2,12 = 8.817; p = 0.004. WT Sham vs Tg Casp3: * p = 0.011, Tg Sham vs Tg Casp3: ** p = 0.007, with Tukey’s). The insets and bottom plot show AT8-reactive cells and AT8 levels (scale: 20 µm; n = 5 mice/group. One-Way ANOVA: F 2,12 = 19.27; p = 0.0002. WT vs Tg Casp3: *** p = 0.0003, Tg Sham vs Tg Casp3: *** p = 0.0007, with Tukey’s). H MAP2 immunostaining and plot showing MAP2 levels (counterstained with NeuroTrace; scale: 25 µm; Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t -test: * p = 0.017). I 6E10 immunostaining and plot of intracellular Aβ levels (Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t -test: ** p = 0.002). Nuclei are counterstained with DAPI (scale: 50 µm). J) Immunostaining for Aβ plaques and Iba1 (scale: 100 µm, inset 20 µm), and plot reporting hippocampal Aβ plaque load ( n = 7 mice/group; Mann–Whitney test: * p = 0.021). K DA and 5-HT loss due to midbrain lesion triggers microglia-astrocyte-neuron crosstalk fostering neuroinflammation and, thus, exacerbating AD pathology in the hippocampus of Tg Casp3 mice. [Figure created using BioRender.com]
Rat Anti C3ar, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti rat c3a receptor c3ar  (Hycult Biotech)
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DAF treatment reduces interaction of <t>C3a-C3aR</t> in the rat brain tissue after blast exposure. Representative photomicrographs of C3a-C3aR interaction in frontal grey matter ( a ) and hippocampus (DG) ( b ) of frozen sections stained with anti-C3a (red) and anti-C3aR (green) antibodies. Original magnification of × 200 (grey matter) and × 400 (DG). Scale bars, 200 μm (grey matter) and 100 μm (DG). n = 8 for control, 3 and 24 h experimental groups. n = 5 for 48 h experimental groups.
Mouse Anti Rat C3a Receptor C3ar, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hm3028  (Hycult Biotech)
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DAF treatment reduces interaction of <t>C3a-C3aR</t> in the rat brain tissue after blast exposure. Representative photomicrographs of C3a-C3aR interaction in frontal grey matter ( a ) and hippocampus (DG) ( b ) of frozen sections stained with anti-C3a (red) and anti-C3aR (green) antibodies. Original magnification of × 200 (grey matter) and × 400 (DG). Scale bars, 200 μm (grey matter) and 100 μm (DG). n = 8 for control, 3 and 24 h experimental groups. n = 5 for 48 h experimental groups.
Hm3028, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+c3ar/pm36104755-108-64-65?v=Hycult+Biotech
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Reactive astrocytes, tau and Aβ pathology in Tg Casp3 mice. A Stereological cell count of GFAP + cells in the hippocampus (scale: 50 µm) of Tg Sham and Tg Casp3 male mice ( n = 4 mice/group; Mann–Whitney test: * p = 0.029). Nuclei are counterstained with DAPI. B IL-18R and GFAP immunostaining (scale: 15 µm), and plot showing % of IL-18R + /GFAP + cells (Tg Sham: n = 6, Tg Casp3: n = 4 mice; Unpaired t-test: **** p < 0.0001). C p-NFκB and S100β immunostaining (scale: 15 µm) and plot showing % of p-NFκB + /S100β + cells in hippocampus (Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t-test: **** p < 0.0001). D Complement C3 and GFAP immunostaining (scale: 10 µm), and plot showing C3 levels ( n = 5 mice/group; Unpaired t -test: ** p = 0.001). E Complement C3aR and DAPI (scale: 20 µm) or Iba1 immunostaining (scale: 20 µm, inset 10 µm), and plots showing C3aR levels on neurons ( n = 4 mice/group; Unpaired t -test: *** p = 0.0007) and microglia (Unpaired t -test: *** p = 0.0003). F Representative western blots and plot showing levels of p-GSK3β/GSK3β (as % of Tg Sham). β-Tubulin was used as loading control ( n = 6 mice/group; Unpaired t -test: * p = 0.048). G AT8 immunostaining in WT, Tg Sham and Tg Casp3 hippocampi and plot showing AT8 + area on total hippocampal area (scale bar: 250 µm; n = 5 mice/group. One-Way ANOVA: F 2,12 = 8.817; p = 0.004. WT Sham vs Tg Casp3: * p = 0.011, Tg Sham vs Tg Casp3: ** p = 0.007, with Tukey’s). The insets and bottom plot show AT8-reactive cells and AT8 levels (scale: 20 µm; n = 5 mice/group. One-Way ANOVA: F 2,12 = 19.27; p = 0.0002. WT vs Tg Casp3: *** p = 0.0003, Tg Sham vs Tg Casp3: *** p = 0.0007, with Tukey’s). H MAP2 immunostaining and plot showing MAP2 levels (counterstained with NeuroTrace; scale: 25 µm; Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t -test: * p = 0.017). I 6E10 immunostaining and plot of intracellular Aβ levels (Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t -test: ** p = 0.002). Nuclei are counterstained with DAPI (scale: 50 µm). J) Immunostaining for Aβ plaques and Iba1 (scale: 100 µm, inset 20 µm), and plot reporting hippocampal Aβ plaque load ( n = 7 mice/group; Mann–Whitney test: * p = 0.021). K DA and 5-HT loss due to midbrain lesion triggers microglia-astrocyte-neuron crosstalk fostering neuroinflammation and, thus, exacerbating AD pathology in the hippocampus of Tg Casp3 mice. [Figure created using BioRender.com]

Journal: Molecular Neurodegeneration

Article Title: Midbrain degeneration triggers astrocyte reactivity and tau pathology in experimental Alzheimer’s Disease

doi: 10.1186/s13024-025-00893-2

Figure Lengend Snippet: Reactive astrocytes, tau and Aβ pathology in Tg Casp3 mice. A Stereological cell count of GFAP + cells in the hippocampus (scale: 50 µm) of Tg Sham and Tg Casp3 male mice ( n = 4 mice/group; Mann–Whitney test: * p = 0.029). Nuclei are counterstained with DAPI. B IL-18R and GFAP immunostaining (scale: 15 µm), and plot showing % of IL-18R + /GFAP + cells (Tg Sham: n = 6, Tg Casp3: n = 4 mice; Unpaired t-test: **** p < 0.0001). C p-NFκB and S100β immunostaining (scale: 15 µm) and plot showing % of p-NFκB + /S100β + cells in hippocampus (Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t-test: **** p < 0.0001). D Complement C3 and GFAP immunostaining (scale: 10 µm), and plot showing C3 levels ( n = 5 mice/group; Unpaired t -test: ** p = 0.001). E Complement C3aR and DAPI (scale: 20 µm) or Iba1 immunostaining (scale: 20 µm, inset 10 µm), and plots showing C3aR levels on neurons ( n = 4 mice/group; Unpaired t -test: *** p = 0.0007) and microglia (Unpaired t -test: *** p = 0.0003). F Representative western blots and plot showing levels of p-GSK3β/GSK3β (as % of Tg Sham). β-Tubulin was used as loading control ( n = 6 mice/group; Unpaired t -test: * p = 0.048). G AT8 immunostaining in WT, Tg Sham and Tg Casp3 hippocampi and plot showing AT8 + area on total hippocampal area (scale bar: 250 µm; n = 5 mice/group. One-Way ANOVA: F 2,12 = 8.817; p = 0.004. WT Sham vs Tg Casp3: * p = 0.011, Tg Sham vs Tg Casp3: ** p = 0.007, with Tukey’s). The insets and bottom plot show AT8-reactive cells and AT8 levels (scale: 20 µm; n = 5 mice/group. One-Way ANOVA: F 2,12 = 19.27; p = 0.0002. WT vs Tg Casp3: *** p = 0.0003, Tg Sham vs Tg Casp3: *** p = 0.0007, with Tukey’s). H MAP2 immunostaining and plot showing MAP2 levels (counterstained with NeuroTrace; scale: 25 µm; Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t -test: * p = 0.017). I 6E10 immunostaining and plot of intracellular Aβ levels (Tg Sham: n = 6, Tg Casp3: n = 5 mice; Unpaired t -test: ** p = 0.002). Nuclei are counterstained with DAPI (scale: 50 µm). J) Immunostaining for Aβ plaques and Iba1 (scale: 100 µm, inset 20 µm), and plot reporting hippocampal Aβ plaque load ( n = 7 mice/group; Mann–Whitney test: * p = 0.021). K DA and 5-HT loss due to midbrain lesion triggers microglia-astrocyte-neuron crosstalk fostering neuroinflammation and, thus, exacerbating AD pathology in the hippocampus of Tg Casp3 mice. [Figure created using BioRender.com]

Article Snippet: Primary antibodies: 5-HT (1:500; ImmunoStar #20080; RRID:AB_572263), AT8 Ser202/Thr205 (1:200; Invitrogen #1020; RRID:AB_223647), C3 (1:300; Novus Biologicals #NB200-540; RRID:AB_2744548), C3aR (1:200; HycultBiotech #HM3028; RRID:AB_2131309), CD68 (1:400; Biorad #MCA1957; RRID:AB_322219), DAT (1:400; Chemicon #MAB369; RRID:AB_2190413), GFAP (1:1000; Millipore #AB5804; RRID:AB_2109645), GFAP (1:1000; DAKO #Z0334; RRID:AB_2314535), hAPP695 (6E10) (1:500; BioLegend #803001; RRID: AB_2564653), Iba1 (1:600; Wako #019–19741; RRID:AB_839504), AIF-1/Iba1 (1:600; Novus Biologicals #NB100-1028; RRID:AB_3148646), IL-1β (1:200; R&D #AF-401-NA; RRID:AB_416684), IL-18 (1:300; MBL #D047-3; RRID:AB_592016), IL-18R⍺/IL-1 r5 (1:100; R&D #AF856; RRID:AB_355664), MAP2 (1:500; Invitrogen #MA5-12,826; RRID:AB_10976831), NET (1:500; Atlas Antibodies #AMAb91116; RRID:AB_2665806), NLRP3 (1:200; Adipogen #AG-20B-0014; RRID:AB_2490202), p-NFκB (Ser536) (1:200; Cell Signaling #3033; RRID:AB_331284), S100β (1:500; SYSY #287 006; RRID:AB_2713986), SERT (1:500; Millipore #PC177L; RRID:AB_2122553), TH (1:1000; Millipore #MAB318; RRID:AB_2201528), TH (1:500; Millipore #AB152; RRID: AB_390204).

Techniques: Cell Counting, MANN-WHITNEY, Immunostaining, Western Blot, Control

DAF treatment reduces interaction of C3a-C3aR in the rat brain tissue after blast exposure. Representative photomicrographs of C3a-C3aR interaction in frontal grey matter ( a ) and hippocampus (DG) ( b ) of frozen sections stained with anti-C3a (red) and anti-C3aR (green) antibodies. Original magnification of × 200 (grey matter) and × 400 (DG). Scale bars, 200 μm (grey matter) and 100 μm (DG). n = 8 for control, 3 and 24 h experimental groups. n = 5 for 48 h experimental groups.

Journal: Acta Neuropathologica Communications

Article Title: Protective effects of decay-accelerating factor on blast-induced neurotrauma in rats

doi: 10.1186/2051-5960-1-52

Figure Lengend Snippet: DAF treatment reduces interaction of C3a-C3aR in the rat brain tissue after blast exposure. Representative photomicrographs of C3a-C3aR interaction in frontal grey matter ( a ) and hippocampus (DG) ( b ) of frozen sections stained with anti-C3a (red) and anti-C3aR (green) antibodies. Original magnification of × 200 (grey matter) and × 400 (DG). Scale bars, 200 μm (grey matter) and 100 μm (DG). n = 8 for control, 3 and 24 h experimental groups. n = 5 for 48 h experimental groups.

Article Snippet: Mouse anti-rat C3a receptor (C3aR) and mouse anti-rat C5b-9 antibodies were acquired from Hycult Biotech Inc (Plymouth Meeting, PA).

Techniques: Staining